US President Barack Obama has said the use of chemical weapons by Syria would be a "red line" that would change his thinking on intervention in the crisis.
He said he had "at this point not ordered military engagement".
But he added: "There would be enormous consequences if we start seeing movement on the chemical weapons front or the use of chemical weapons."
Earlier the new UN special envoy to Syria faced criticism for refusing to say whether President Assad must quit.
President Obama, speaking to reporters at a White House briefing, said the deployment or use of biological weapons would widen the conflict in the region.
He said: "It doesn't just include Syria. It would concern allies in the region, including Israel, and it would concern us."
He warned President Bashar al-Assad and "other players on the ground" about the use or movement of such weapons.
He said: "A red line for us is [if] we see a whole bunch of chemical weapons moving around, or being utilised. That would change my calculus."
Syria holds the world's fourth-largest stockpile of chemical weapons. Last month a Syrian foreign ministry spokesman said the weapons would never be deployed inside Syria.
However, the BBC's Kim Ghattas in Washington says the US has seen unconfirmed reports recently that the Syrian authorities have been moving the country's chemical arms stockpile.
Mission endsEarlier on Monday, the UN's new envoy to Syria, Lakhdar Brahimi told the BBC that he was "not in a position to say yet" whether President Assad should go, but was "committed to finding a solution".
Mr Brahimi, a former Algerian foreign minister, last week succeeded Kofi Annan who resigned after both sides largely ignored his peace plan.
On Sunday, UN observers ended their mission to verify its implementation.
Their departure came after the UN Security Council agreed to allow their mandate to expire at midnight, and instead set up a new civilian office in Damascus to pursue political contacts that might lead to peace.
Since being confirmed as the new UN and Arab League envoy to Syria, Mr Brahimi has acknowledged that he has no concrete ideas of how to end the conflict, which he believes has been a civil war for some time.
On Monday, he told the BBC that he was not ready to say whether President Assad should step down despite widespread international condemnation of his government's crackdown on dissent since protests erupted in March 2011.
"I am not in a position to say yet, because I was appointed a couple of days ago. I am going to New York for the first time to see the people who I am going to work for, and I am going to Cairo see the Arab League," he explained.
After announcing his resignation, Mr Brahimi's predecessor, Kofi Annan, said: "It is clear that President Bashar al-Assad must leave office."
The main opposition coalition, the Syrian National Council (SNC), said Mr Brahimi's stance showed "disregard for the blood of the Syrian people and their right of self-determination" and demanded he apologise.
Mr Brahimi stressed that he was "committed to finding a solution full stop".
"I am a mediator. I haven't joined any Syrian party. I am a mediator and a mediator has to speak to anybody and everybody without influence or interest," he added.
"Then I'll make up my mind about what to say and what to do."
'Shellfire'On the ground in Syria, seven UN vehicles drove out of the capital on Monday morning, carrying some of the last members of the UN Supervision Mission in Syria (UNSMIS), all of whom are expected to leave the country by the end of the week.
As they departed, government forces were trying to retake the Damascus suburb of Muadhamiya from rebel fighters, opposition activists said. One report said seven people had been killed by shellfire.
The UK-based opposition activist group, the Syrian Observatory for Human Rights, said fighting in the southern city of Deraa had left 15 people dead, including two children.
Shelling by tanks and warplanes also caused houses to collapse in the northern city of Aleppo, killing at least 14 people, activist Mohammed Saeed told the Associated Press. The buildings were in the Sakhour and Qadi Askar districts, he said.
The UN says more than 18,000 people have been killed in the conflict, 170,000 have fled Syria and 2.5 million need aid within the country.
Source: http://www.bbc.co.uk/news/world-middle-east-19319446#sa-ns_mchannel=rss&ns_source=PublicRSS20-sa
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